Fermentation Biotechnologist
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12. ED. QUALIFICATION : M.Sc. Microbiology
Univ. / Board Passing Year Percentage / Grade
M. Sc. Microbiology Shivaji Univ. 1992 66.38%
B. Sc. Microbiology Shivaji Univ. 1990 72.68%
H. S. C. Pune Board 1987 53.00%
S. S. C. Pune Board 1984 77.42%
Current Job Profile and Experience
1. BANGALORE (Since Oct. 2007)
In the current organization I am working as Manager – Fermentation heading the Fermentation Department.
Our company is primarily an engineering company involved in the production of complete biopharmaceutical production facilities viz. fermentors, bioreactors & related equipments along with the automation on a turn key basis. We also provide complete consultancy for the biopharmaceutical production and also provide training in the field of specialized services for the biotech world. The production and consultancy involve design, build and delivery of biotech facility. The training part involves theory and practicals, involving intensive hands on experience to enrich technical and operational skills of the participants.
As a head of fermentation department I am responsible for all process related support for the production and consultancy projects. Similarly, I am responsible for complete training activities of fermentation involving intensive theory and practical programmes for participants coming from industry and colleges.
Using the pilot production facility in conjunction with the laboratory facility, we also undertake project work for developmental work for the bio-pharmaceutical customers.
2. MERIND LTD., A WOCKHARDT ENTERPRISE, MUMBAI
(Oct. 2001 TO Aug. 2006)
In my previous engagement I was In-charge of Pilot Fermentation attached to Vitamin B12 production facility.
Primarily, I oversaw the pilot group activities involving planning of R&D experimental batches; execution, repairs and maintenance follow-up, raw material and spares inventory upkeep.
Simultaneously, in the upstream production work I was responsible for culture maintenance, inoculum buildup for the production batches, contamination, other troubleshooting, scale-up studies and developmental studies.
3. NICHOLAS PIRAMAL INDIA LTD, MUMBAI
Executive, R&D, Lab/Pilot Fermentation (Oct. 1998 TO Oct. 2001)
4. HOECHST MARION ROUSSEL LTD, MUMBAI
Research Associate, got promoted to Executive, R&D, Lab/Pilot Fermentation (Apr. 1994 TO Sep. 1998)
5. MICROBIOLOGY DEPT, POONA UNIVERSITY, PUNE
Junior Research Fellow on a Department of Biotechnology project with Amrut Biotech/Poona University joint project (Feb. 1994 TO Apr. 1994)
6. RANBAXY LABS. LTD., NEW DELHI
SHIFT SUPERVISOR in R&D, fermentation laboratory (Mar. 1993 TO Oct. 1993)
7. LUPIN CHEMICALS, TARAPUR
Tr. Production Officer in production (Jan. 1993 TO Mar. 1993)
Different work responsibilities / job expertise involve
- Departmental work planning, delegation, implementation to achieve yearly targets.
- Expert in fermentation operations from culture preservation to inoculum build-up, vessel and media sterilizations and cleaning (ESIP, FSIP & CIP), inoculum and seed sterile transfers, parameter monitoring, contamination control.
- Can handle manual as well as automatic control fermentor units from lab level to as big as 100KL capacity.
- Testing new raw materials at shake flask and pilot fermentor level.
- Optimization of media components for yield improvement & cost reduction.
- Microbiological analysis of samples for sterility testing and phage monitoring.
- Installation and optimization of new vessel parameters to match the desired fermentation process.
- Basic operations of HPLC analysis.
- Monitoring and trouble shooting of US related production activities.
- Validation and calibration of laboratory instruments.
- Complete data upkeep (hard and soft) of R&D experiments including shake flask experiments, pilot plant and production trials.
- Documentation, SOP preparation and implementation.
- International audit exposure (European)
- Manpower training.
- Specialist to address “Upstream contamination & productivity” problem.
ACHIEVEMENTS:
Current Organization
1. Successfully supported fermentation related production and consultancy projects.
2. Successfully completed streptokinase developmental project work for customer.
3. Successfully completed algal fermentation scale-up developmental project work for customer.
4. Streamlined fermentation training activities with complete series of presentations and practicals.
Merind
1. Streamlined pilot fermentor operations of Vit B12 to achieve sterility and reproducibility of experiments.
2. Trained the fresh recruits & colleagues in complete fermentation operations.
3. Brought down the contamination rate to almost zero percent.
4. Got new fermentor vessels installed, operationalized and streamlined for smooth operations, along with SOP preparation and manpower training.
5. Achieved the reproducibility of experiments by implementing SOPs / GLPs.
6. Achieved yield improvements (up to 22%) in general and with different experimentation with the different raw materials.
7. Setup efficient procedure for sterility testing of pilot and plant samples at different levels to pinpoint contamination source.
8. Effectively tackled contamination problem in the production unit.
9. Envisaged a definite phage attack and suggested an efficient control strategy for long-term sustenance well in advance.
10. Also, carried out very important and sensitive pilot scale experiments in line with phage attack prevention strategy successfully and analyzed them accurately to conclude the efficacy of the same.
Nicholas Piramal Ltd. – Hoechst Marion Roussel Ltd.
1. Successfully handled fermentation activities of the basic R&D facility consisting of a battery of 18 fermentors (lab to pilot scale, manual – semi-automated).
2. Part of the group responsible for discovery of many new lead molecules, some of which later developed into therapeutically active pharma products.
3. One group patent for a pharmaceutically active base molecule (HMR, Frankfurt)
Ranbaxy Labs Pvt. Ltd
1. Part of the group responsible for successful technology transfer for cephalosporin – C, responsible for complete upstream and downstream lab activities.
# I have hands on experience of bacterial, yeast (including GMO) and fungal and to some extent animal cell culture fermentation.
# I am well conversant with computers – MS-Office, Word, Excel, Lotus Notes, and Internet.
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